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UF Health University of Florida
The Makino Lab The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology
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Research Overview

Publications

  1. Research Articles
  2. Review Articles
  3. Methodological Articles

Research Article

Coronary Microvascular Disease and Coronary Endothelial Dysfunction in Diabetes

  1. Gao L, Ramirez FJ, Cabrera JTO, Varghese MV, Watanabe M, Tsuji-Hosokawa A, Zheng Q, Yang M, Razan MR, Kempf CL, Camp SM, Wang J, Garcia JGN, Makino A. eNAMPT is a novel therapeutic target for mitigation of coronary microvascular disease in type 2 diabetes. Diabetologia 67(9):1998-2011. 2024.
  2. Cabrera JT, Si R, Tsuji-Hosokawa A, Cai H, Yuan JX-J, Dillmann WH, Makino A. Restoration of coronary microvascular function by OGA overexpression in a high-fat diet with low-dose streptozotocin-induced type 2 diabetic mice. Diab. Vasc. Dis. Res. 20(3):14791641231173630. 2023.
  3. Si R, Cabrera JTO, Tsuji-Hosokawa A, Guo R, Watanabe M, Gao L, Lee YS, Moon JS, Scott BT, Wang J, Ashton AW, Rao JN, Wang JY, Yuan JX-J, Makino A. HuR/Cx40 downregulation causes coronary microvascular dysfunction in type 2 diabetes. JCI Insight 6(21):e147982. 2021.
  4. Si R, Zhang Q, Tsuji-Hosokawa A, Watanabe M, Willson C, Lai N, Wang J, Dai A, Scott BT, Dillmann WH, Yuan JX, Makino A. Overexpression of p53 due to excess protein O-GlcNAcylation is associated with coronary microvascular disease in type 2 diabetes. Cardiovasc. Res. 116(6):1186-1198. 2020.
  5. Zhang Q, Tsuji-Hosokawa A, Willson C, Watanabe M, Si R, Lai N, Wang Z, Yuan JX, Wang J, Makino A. Chloroquine differentially modulates coronary vascular relaxation in control and diabetic mice. Br. J. Pharmacol. 177(2):314-327. 2020.
  6. Pan M, Han Y, Basu A, Dai A, Si R, Willson C, Balistrieri A, Scott BT, Makino A. Overexpression of hexokinase 2 reduces mitochondrial calcium overload in coronary endothelial cells of type 2 diabetic mice. Am. J. Physiol. Cell. Physiol. 314(6):C732-C740. 2018.
  7. Guo R, Si R, Scott BT, Makino A. Mitochondrial connexin40 regulates mitochondrial calcium uptake in coronary endothelial cells. Am. J. Physiol. Cell. Physiol. 312(4):C398-C406. 2017.
  8. Han Y, Cho YE, Ayon R, Guo R, Youssef KD, Pan M, Dai A, Yuan JX, Makino A. SGLT inhibitors attenuate NO-dependent vascular relaxation in the pulmonary artery but not in the coronary artery. Am. J. Physiol. Lung Cell. Mol. Physiol. 309(9):L1027-L1036. 2015.
  9. Makino A, Dai A, Han Y, Youssef KD, Wang W, Donthamsetty R, Scott BT, Wang H, Dillmann WH. O-GlcNAcase overexpression reverses coronary endothelial cell dysfunction in type 1 diabetic mice. Am. J. Physiol. Cell Physiol. 309(9):C593-C599. 2015.
  10. Cho YE, Basu A, Dai A, Heldak M, Makino A. Coronary endothelial dysfunction and mitochondrial reactive oxygen species in type 2 diabetic mice. Am. J. Physiol. Cell Physiol. 305(10):C1033-C1040. 2013.
  11. Estrada IA, Donthamsetty R, Debski P, Zhou MH, Zhang SL, Yuan JX, Han W, Makino A. STIM1 restores coronary endothelial function in type1 diabetic mice. Circ. Res. 111(9):1166-1175. 2012.
  12. Sasaki K, Donthamsetty R, Heldak M, Cho YE, Scott BT, Makino A. VDAC: An old protein with new roles in diabetes. Am. J. Physiol. Cell Physiol. 303(10):C1055–C1060. 2012.
  13. Makino A, Scott BT, Dillmann WH. Mitochondrial fragmentation and superoxide anion production in coronary endothelial cells from a mouse model of type 1 diabetes. Diabetologia 53(8):1783-1794. 2010.
  14. Makino A, Platoshyn O, Suarez J, Yuan JX, Dillmann WH. Downregulation of connexin40 is associated with coronary endothelial cell dysfunction in streptozotocin-induced diabetic mice. Am. J. Physiol. Cell Physiol. 295(1):C221-230. 2008.

Cardiovascular Disease in Diabetes

  1. Liu Z, Zhang Y, Youn JY, Zhang Y, Makino A, Yuan JX, Cai H. Flavored and nicotine-containing E-cigarettes induce impaired angiogenesis and diabetic wound healing via increased endothelial oxidative stress and reduced NO bioavailability. Antioxidants (Basel). 11(5):904. 2022.
  2. Sudhahar V, Okur MN, Bagi Z, O’Bryan JP, Hay N, Makino A, Patel VS, Phillips SA, Stepp D, Ushio-Fukai M, Fukai T. Akt2 stabilizes ATP7A, a copper transporter for SOD3 (extracellular superoxide dismutase), in vascular smooth muscles. Novel mechanism to limit endothelial dysfunction in type 2 diabetes mellitus. Arterioscler. Thromb. Vasc. Biol. 38(3):529-541. 2018.
  3. Yue L, Bian JT, Grizelj I, Cavka A, Phillips SA, Makino A, Mazzone T. Apolipoprotein E enhances endothelial-NO production by modulating caveolin 1 interaction with endothelial NO synthase. Hypertension. 60(4):1040-6. 2012.
  4. Suarez J, Hu Y, Makino A, Fricovsky E, Wang H, Dillmann WH. Alterations in mitochondrial function and cytosolic calcium induced by hyperglycemia are restored by mitochondrial transcription factor A in cardiomyocytes. Am. J. Physiol. Cell Physiol. 295(6):C1561-C1568. 2008.
  5. Kamata K, Makino A, Kanie N, Oda S, Matsumoto T, Kobayashi T, Kikuchi T, Nishimura M, Honda T. Effects of anthocyanidin derivative (HK-008) on relaxation in rat perfused mesenterial bed. J. Smooth Muscle Res. 42:75-88. 2006.
  6. Makino A. Effect of chronic administration of L-arginine on vasoactive responses induced by endothelin-1 and its plasma level in streptozotocin-induced diabetic rats. J. Smooth Muscle Res. 38:101-115. 2002.
  7. Makino A, Oda S, Kamata K. Mechanisms underlying increased release of endothelin-1 from aorta in diabetic rats. Peptides. 2001 Apr;22(4):639-45. doi: 10.1016/s0196-9781(01)00374-6. PMID: 11311735.
  8. Makino A, Ohuchi K, Kamata K. Time-course changes in plasma endothelin-1 and its effects on the mesenteric arterial bed in streptozotocin-induced diabetic rats. Diabetes, Obesity and Metabolism. 2(1):47-55. 2000.
  9. Makino A, Ohuchi K, Kamata K. Mechanism underlying the attenuation of endothelium-dependent vasodilatation in the mesenteric arterial bed of the streptozotocin-induced diabetic rats. Br. J. Pharmacol. 130:549-556. 2000.
  10. Makino A, Kamata K. Possible modulation by endothelin-1, nitric oxide, prostaglandin I2 and thromboxane A2 of vasoconstriction induced by an α-agonist in mesenteric arterial bed from diabetic rats. Diabetologia. 41:1410-1418. 1998.
  11. Makino A, Kamata K. Elevated plasma endothelin-1 level in streptozotocin-induced diabetic rats and responsiveness of the mesenteric arterial bed to endothelin-1. Br. J. Pharmacol. 123:1065-1072. 1998.
  12. Kamata K, Makino A. A comparative study on the rat aorta and mesenteric arterial bed of the possible role of nitric oxide in the desensitization of the vasoconstrictor response to an α1-adrenoceptor agonist. Br. J. Pharmacol. 120:1221-1228. 1997.

Pulmonary Hypertension and Pulmonary Vascular Disease

  1. Ahmed M, Kee SL, Zaghloul N, Casanova NG, Rodriguez M, Kempf CL, Camp SM, Song JH, Sammani S, Gupta A, Bryant AJ, Ooi A, Desai AA, Makino A, Yuan JX, Benos PV, Dai Z, Garcia JGN. eNAMPT/TLR4 signaling drives PAH cellular phenotypic switching and lung vascular remodeling. Cell Commun Signal. 23(1):465. 2025. PMCID: PMC12573901.
  2. Babicheva A, Elmadbouh I, Song S, Thompson MA, Powers R, Jain PP, Izadi A, Chen J, Yung L, Parmisano S, Paquin C, Wang WT, Chen Y, Wang T, Alotaibi M, Shyy JY, Thistlethwaite PA, Wang J, Makino A, Prakash YS, Pabelick CM, Yuan JX. Store-operated Ca2+ entry is involved in endothelium-to-mesenchymal transition in lung vascular endothelial cells. Am J Physiol Lung Cell Mol Physiol. 328(6):L844-L857. 2025. PMCID: PMC12169631.
  3. Zheng Q, Cabrera JTO, Tsuji-Hosokawa A, Ramirez FJ, Cai H, Yuan JX, Wang J, Makino A. Enhanced lung endothelial glycolysis is implicated in the development of severe pulmonary hypertension in type 2 diabetes. Am J Physiol Lung Cell Mol Physiol. 328(3):L430-L442. 2025. PMCID: PMC12186236.
  4. Zhao T, Parmisano S, Soroureddin Z, Zhao M, Yung L, Thistlethwaite PA, Makino A, Yuan JX. Mechanosensitive cation currents through TRPC6 and Piezo1 channels in human pulmonary arterial endothelial cells. Am J Physiol Cell Physiol. 323(4):C959-C973. 2022. PMCID: PMC9485000.
  5. Jain PP, Hosokawa S, Babicheva A, Zhao T, Chen J, Thistlethwaite PA, Makino A, Yuan JX. In vivo and ex vivo experimental approach for studying functional role of notch in pulmonary vascular disease. Methods Mol Biol. 2472:209-220. 2022. PMID: 35674903.
  6. Chen J, Rodriguez M, Miao J, Liao J, Jain PP, Zhao M, Zhao T, Babicheva A, Wang Z, Parmisano S, Powers R, Matti M, Paquin C, Soroureddin Z, Shyy JY, Thistlethwaite PA, Makino A, Wang J, Yuan JX. Mechanosensitive channel Piezo1 is required for pulmonary artery smooth muscle cell proliferation. Am J Physiol Lung Cell Mol Physiol. 322(5):L737-L760. 2022. PMCID: PMC9076422.
  7. Ahmed M, Zaghloul N, Zimmerman P, Casanova NG, Sun X, Song JH, Hernon VR, Sammani S, Rischard F, Rafikova O, Rafikov R, Makino A, Kempf CL, Camp SM, Wang J, Desai AA, Lussier Y, Yuan JX, Garcia JGN. Endothelial eNAMPT drives EndMT and preclinical PH: rescue by an eNAMPT-neutralizing mAb. Pulm Circ. 11(4):20458940211059712. 2021. PMCID: PMC8591779.
  8. Jain PP, Lai N, Xiong M, Chen J, Babicheva A, Zhao T, Parmisano S, Zhao M, Paquin C, Matti M, Powers R, Balistrieri A, Kim NH, Valdez-Jasso D, Thistlethwaite PA, Shyy JY, Wang J, Garcia JGN, Makino A, Yuan JX. TRPC6, a therapeutic target for pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1;321(6):L1161-L1182. doi: 10.1152/ajplung.00159.2021. Epub 2021 Oct 27. PMID: 34704831; PMCID: PMC8715021.
  9. Wang Z, Chen J, Babicheva A, Jain PP, Rodriguez M, Ayon RJ, Ravellette KS, Wu L, Balistrieri F, Tang H, Wu X, Zhao T, Black SM, Desai AA, Garcia JGN, Sun X, Shyy JY, Valdez-Jasso D, Thistlethwaite PA, Makino A, Wang J, Yuan JX. Endothelial upregulation of mechanosensitive channel Piezo1 in pulmonary hypertension. Am J Physiol Cell Physiol. 321(6):C1010-C1027. 2021. PMCID: PMC8714987.
  10. Rodriguez M, Chen J, Jain PP, Babicheva A, Xiong M, Li J, Lai N, Zhao T, Hernandez M, Balistrieri A, Parmisano S, Simonson T, Breen E, Valdez-Jasso D, Thistlethwaite PA, Shyy JY, Wang J, Garcia JGN, Makino A, Yuan JX. Upregulation of calcium homeostasis modulators in contractile-to-proliferative phenotypical transition of pulmonary arterial smooth muscle cells. Front Physiol. 12:714785. 2021. PMCID: PMC8364962.
  11. Xiong M, Jain PP, Chen J, Babicheva A, Zhao T, Alotaibi M, Kim NH, Lai N, Izadi A, Rodriguez M, Li J, Balistrieri A, Balistrieri F, Parmisano S, Sun X, Valdez-Jasso D, Shyy JY, Thistlethwaite PA, Wang J, Makino A, Yuan JX. Mouse model of experimental pulmonary hypertension: Lung angiogram and right heart catheterization. Pulm Circ. 11(4):20458940211041512. 2021. PMCID: PMC8438952.
  12. Jain PP, Zhao T, Xiong M, Song S, Lai N, Zheng Q, Chen J, Carr SG, Babicheva A, Izadi A, Rodriguez M, Rahimi S, Balistrieri F, Rahimi S, Simonson T, Valdez-Jasso D, Thistlethwaite PA, Shyy JY, Wang J, Makino A, Yuan JX. Halofuginone, a promising drug for treatment of pulmonary hypertension. Br J Pharmacol. 178(17):3373-3394. 2021. PMCID: PMC9792225.
  13. Liao J, Lu W, Chen Y, Duan X, Zhang C, Luo X, Lin Z, Chen J, Liu S, Yan H, Chen Y, Feng H, Zhou D, Chen X, Zhang Z, Yang Q, Liu X, Tang H, Li J, Makino A, Yuan JX, Zhong N, Yang K, Wang J. Upregulation of Piezo1 (Piezo type mechanosensitive ion channel component 1) enhances the intracellular free calcium in pulmonary arterial smooth muscle cells from idiopathic pulmonary arterial hypertension patients. Hypertension. 77(6):1974-1989. 2021. PMID: 33813851.
  14. Si R, Zhang Q, Cabrera JTO, Zheng Q, Tsuji-Hosokawa A, Watanabe M, Hosokawa S, Xiong M, Jain PP, Ashton AW, Yuan JX, Wang J, Makino A. Chronic Hypoxia Decreases Endothelial Connexin 40, Attenuates Endothelium-Dependent Hyperpolarization-Mediated Relaxation in Small Distal Pulmonary Arteries, and Leads to Pulmonary Hypertension. J Am Heart Assoc. 2020 Dec 15;9(24):e018327. doi: 10.1161/JAHA.120.018327. Epub 2020 Dec 12. PMID: 33307937; PMCID: PMC7955394.
  15. Romanoski CE, Qi X, Sangam S, Vanderpool RR, Stearman RS, Conklin A, Gonzalez-Garay M, Rischard F, Ayon RJ, Wang J, Simonson T, Babicheva A, Shi Y, Tang H, Makino A, Kanthi Y, Geraci MW, Garcia JGN, Yuan JX, Desai AA. Transcriptomic profiles in pulmonary arterial hypertension associate with disease severity and identify novel candidate genes. Pulm Circ. 10(4):2045894020968531. 2020. PMCID: PMC7727059.
  16. Jain PP, Hosokawa S, Xiong M, Babicheva A, Zhao T, Rodriguez M, Rahimi S, Pourhashemi K, Balistrieri F, Lai N, Malhotra A, Shyy JY, Valdez-Jasso D, Thistlethwaite PA, Makino A, Yuan JX. Revisiting the mechanism of hypoxic pulmonary vasoconstriction using isolated perfused/ventilated mouse lung. Pulm Circ. 10(4):2045894020956592. 2020. PMCID: PMC7691930.
  17. Zhu Z, Wang Y, Long A, Feng T, Ocampo M, Chen S, Tang H, Guo Q, Minshall R, Makino A, Huang W, Chen J. Pulmonary vessel casting in a rat model of monocrotaline-mediated pulmonary hypertension. Pulm Circ. 10(3):2045894020922129. 2020. PMCID: PMC7448137.
  18. Wu K, Tang H, Lin R, Carr SG, Wang Z, Babicheva A, Ayon RJ, Jain PP, Xiong M, Rodriguez M, Rahimi S, Balistrieri F, Rahimi S, Valdez-Jasso D, Simonson TS, Desai AA, Garcia JGN, Shyy JY, Thistlethwaite PA, Wang J, Makino A, Yuan JX. Endothelial platelet-derived growth factor-mediated activation of smooth muscle platelet-derived growth factor receptors in pulmonary arterial hypertension. Pulm Circ. 10(3):2045894020948470. 2020. PMCID: PMC7707860.
  19. Song S, Babicheva A, Zhao T, Ayon RJ, Rodriguez M, Rahimi S, Balistrieri F, Harrington A, Shyy JY, Thistlethwaite PA, Makino A, Yuan JX. Notch enhances Ca2+ entry by activating calcium-sensing receptors and inhibiting voltage-gated K+ channels. Am J Physiol Cell Physiol. 318(5):C954-C968. 2020. PMCID: PMC7294324.
  20. Zhang C, Zhang T, Lu W, Duan X, Luo X, Liu S, Chen Y, Li Y, Chen J, Liao J, Zhou D, Chen X, Feng H, Gu G, Wang T, Tang H, Makino A, Zhong N, Yuan JX, Yang K, Wang J. Altered airway microbiota composition in patients with pulmonary hypertension. Hypertension. 76(5):1589-1599. 2020. PMID: 32921193.
  21. Chen Y, Lu W, Yang K, Duan X, Li M, Chen X, Zhang J, Kuang M, Liu S, Wu X, Zou G, Liu C, Hong C, He W, Liao J, Hou C, Zhang Z, Zheng Q, Chen J, Zhang N, Tang H, Vanderpool RR, Desai AA, Rischard F, Black SM, Garcia JGN, Makino A, Yuan JX, Zhong N, Wang J. Tetramethylpyrazine: A promising drug for the treatment of pulmonary hypertension. Br J Pharmacol. 2020 Jun;177(12):2743-2764. doi: 10.1111/bph.15000. Epub 2020 Apr 27. PMID: 31976548; PMCID: PMC7236078.
  22. Babicheva A, Ayon RJ, Zhao T, Ek Vitorin JF, Pohl NM, Yamamura A, Yamamura H, Quinton BA, Ba M, Wu L, Ravellette KS, Rahimi S, Balistrieri F, Harrington A, Vanderpool RR, Thistlethwaite PA, Makino A, Yuan JX. MicroRNA-mediated downregulation of K+ channels in pulmonary arterial hypertension. Am J Physiol Lung Cell Mol Physiol. 2020 Jan 1;318(1):L10-L26. doi: 10.1152/ajplung.00010.2019. Epub 2019 Sep 25. PMID: 31553627; PMCID: PMC6985878.
  23. Schwaerzer GK, Kalyanaraman H, Casteel DE, Dalton ND, Gu Y, Lee S, Zhuang S, Wahwah N, Schilling JM, Patel HH, Zhang Q, Makino A, Milewicz DM, Peterson KL, Boss GR, Pilz RB. Aortic pathology from protein kinase G activation is prevented by an antioxidant vitamin B12 analog. Nat Commun. 10(1):3533. 2019. PMCID: PMC6684604.
  24. Wang Z, Yang K, Zheng Q, Zhang C, Tang H, Babicheva A, Jiang Q, Li M, Chen Y, Carr SG, Wu K, Zhang Q, Balistrieri A, Wang C, Song S, Ayon RJ, Desai AA, Black SM, Garcia JGN, Makino A, Yuan JX, Lu W, Wang J. Divergent changes of p53 in pulmonary arterial endothelial and smooth muscle cells involved in the development of pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol. 316(1):L216-L228. 2019. PMCID: PMC6383500.
  25. Tang H, Wu K, Wang J, Vinjamuri S, Gu Y, Song S, Wang Z, Zhang Q, Balistrieri A, Ayon RJ, Rischard F, Vanderpool R, Chen J, Zhou G, Desai AA, Black SM, Garcia JGN, Yuan JX, Makino A. Pathogenic Role of mTORC1 and mTORC2 in Pulmonary Hypertension. JACC Basic Transl Sci. 2018 Dec 31;3(6):744-762. doi: 10.1016/j.jacbts.2018.08.009. PMID: 30623134; PMCID: PMC6314964.
  26. Whitaker M, Nair V, Sinari S, Dherange P, Natarajan B, Trutter L, Brittain E, Hemnes A, Austin E, Patel K, Black S, Garcia J, Yuan JX, Vanderpool R, Rischard F, Makino A, Bedrick E, Desai A. Diabetes mellitus associates with increased right ventricular afterload and remodeling in pulmonary arterial hypertension. Am J Med. S0002-9343(18):30091-3. 2018. PMCID: PMC5963998.
  27. He X, Song S, Ayon RJ, Balistrieri A, Black SM, Makino A, Wier WG, Zang WJ, Yuan JX. Hypoxia selectively upregulates cation channels and increases cytosolic [Ca2+] in pulmonary, but not coronary, arterial smooth muscle cells. Am J Physiol Cell Physiol. 314(4):C504-C517. 2018. PMCID: PMC5966788.
  28. Song S, Carr SG, McDermott KM, Rodriguez M, Babicheva A, Balistrieri A, Ayon RJ, Wang J, Makino A, Yuan JX. STIM2 (Stromal Interaction Molecule 2)-mediated increase in resting cytosolic free Ca2+ concentration stimulates PASMC proliferation in pulmonary arterial hypertension. Hypertension. 71(3):518-529. 2018. PMCID: PMC5955710.
  29. Tang H, Babicheva A, McDermott KM, Gu Y, Ayon RJ, Song S, Wang Z, Gupta A, Zhou T, Sun X, Dash S, Wang Z, Balistrieri A, Zheng QY, Cordery AG, Desai AA, Rischard F, Khalpey Z, Wang J, Black SM, Garcia JGN, Makino A, Yuan JX. Endothelial HIF-2α contributes to severe pulmonary hypertension by inducing endothelial-to-mesenchymal transition. Am J Physiol Lung Cell Mol Physiol. 314(2):L256-L275. 2018. PMCID: PMC5866501.
  30. Wu K, Zhang Q, Wu X, Lu W, Tang H, Liang Z, Gu Y, Song S, Ayon RJ, Wang Z, McDermott KM, Balistrieri A, Wang C, Black SM, Garcia JGN, Makino A, Yuan JX, Wang J. Chloroquine is a potent pulmonary vasodilator that attenuates hypoxia-induced pulmonary hypertension. Br J Pharmacol. 174:4155-4172. 2017. PMCID: PMC5659991.
  31. Pan M, Han Y, Si R, Guo R, Desai A, Makino A. Hypoxia-induced pulmonary hypertension in type 2 diabetic mice. Pulm Circ. 2017 Feb 1;7(1):175-185. doi: 10.1086/690206. PMID: 28680577; PMCID: PMC5448524.
  32. Song S, Ayon RJ, Yamamura A, Yamamura H, Dash S, Babicheva A, Tang H, Sun X, Cordery AG, Khalpey ZI, Black SM, Desai AA, Rischard F, McDermott KM, Garcia JG, Makino A, Yuan JX. Capsaicin-induced Ca2+ signaling is enhanced via upregulated TRPV1 channels in pulmonary artery smooth muscle cells from patients with idiopathic PAH. Am J Physiol Lung Cell Mol Physiol. 312(3):L309-L325. 2017. PMCID: PMC5374303.
  33. Tang H, Yamamura A, Yamamura H, Song S, Fraidenburg DR, Chen J, Gu Y, Pohl NM, Zhou T, Jiménez-Pérez L, Ayon RJ, Desai AA, Goltzman D, Rischard F, Khalpey Z, Black SM, Garcia JG, Makino A, Yuan JX. Pathogenic role of calcium-sensing receptors in the development and progression of pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol. 310(9):L846-L859. 2016. PMCID: PMC4867349.
  34. Song S, Jacobson KN, McDermott KM, Reddy SP, Cress AE, Tang H, Dudek SM, Black SM, Garcia JG, Makino A, Yuan JX. ATP promotes cell survival via regulation of cytosolic [Ca2+] and Bcl-2/Bax ratio in lung cancer cells. Am J Physiol Cell Physiol. 310(2):C99-C114. 2016. PMCID: PMC4719033.
  35. Fernandez RA, Wan J, Song S, Smith KA, Gu Y, Tauseef M, Tang H, Makino A, Mehta D, Yuan JX. Upregulated STIM2, TRPC6 and Orai2 contribute to the transition of pulmonary arterial smooth muscle cells from a contractile to proliferative phenotype. Am J Physiol Cell Physiol. 308(8):C581-C593. 2015. PMCID: PMC4398853.
  36. Smith KA, Voiriot G, Tang H, Fraidenburg DR, Song S, Yamamura H, Yamamura A, Guo Q, Wan J, Pohl NM, Tauseef M, Bodmer R, Ocorr K, Thistlethwaite PA, Haddad GG, Powell FL, Makino A, Mehta D, Yuan JX. Notch activation of Ca2+ signaling mediates hypoxic pulmonary vasoconstriction and pulmonary hypertension. Am J Respir Cell Mol Biol. 53(3):355-367. 2015. PMCID: PMC4566064.
  37. Tang H, Chen J, Fraidenburg DR, Song S, Sysol JR, Drennan AR, Offermanns S, Ye RD, Bonini MG, Minshall RD, Garcia JG, Machado RF, Makino A, Yuan JX. Deficiency of Akt1, but not Akt2, attenuates the development of pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol. 308(2):L208-L220. 2015. PMCID: PMC4338938.
  38. Wan J, Yamamura A, Zimnicka AM, Voiriot G, Smith KA, Tang H, Ayon RJ, Choudhury MS, Ko EA, Wang J, Wang C, Makino A, Yuan JX. Chronic hypoxia selectively enhances L- and T-type voltage-dependent Ca2+ channel activity in pulmonary artery by upregulating Cav1.2 and Cav3.2. Am J Physiol Lung Cell Mol Physiol. 305(2):L154-L164. 2013. PMCID: PMC3726947.
  39. Ko EA, Wan J, Yamamura A, Zimnicka AM, Yamamura H, Yoo HY, Tang H, Smith KA, Sundivakkam PC, Zeifman A, Ayon RJ, Makino A, Yuan JX. Functional characterization of voltage-dependent Ca2+ channels in mouse pulmonary arterial smooth muscle cells: Divergent effect of ROS. Am J Physiol Cell Physiol. 304(11):C1042-C1052. 2013. PMCID: PMC3677177.
  40. Yamamura A, Yamamura H, Guo Q, Zimnicka AM, Wan J, Ko EA, Smith KA, Pohl NM, Song S, Zeifman A, Makino A, Yuan JX. Dihydropyridine Ca2+ channel blockers increase cytosolic [Ca2+] by activating Ca2+-sensing receptors in pulmonary arterial smooth muscle cells. Circ Res. 112(4):640-650. 2013. PMCID: PMC3642037.
  41. Yamamura A, Guo Q, Yamamura H, Zimnicka AM, Pohl NM, Smith KA, Fernandez RA, Zeifman A, Makino A, Dong H, Yuan JX. Enhanced Ca2+-sensing receptor function in idiopathic pulmonary arterial hypertension. Circ Res. 111(4):469-481. 2012. PMCID: PMC3695473.
  42. Yao W, Mu W, Zeifman A, Lofti M, Remillard CV, Makino A, Perkins DL, Garcia JGN, Yuan JX, Zhang W. Fenfluramine-induced gene dysregulation in human pulmonary artery smooth muscle and endothelial cells. Pulm Circ. 1(3):405-418. 2011. PMCID: PMC3224433.
  43. Song MY, Makino A, Yuan JX. STIM2 contributes to enhanced store-operated Ca2+ entry in pulmonary artery smooth muscle cells from patients with idiopathic pulmonary arterial hypertension. Pulm Circ. 1:84-94. 2011. PMCID: PMC3121304.
  44. Ko EA, Song MY, Donthamsetty R, Makino A, Yuan JX. Tension measurement in isolated rat and mouse pulmonary artery. Drug Discovery Today: Disease Models. 7:123-130. 2010. PMCID: PMC3501740.
  45. Li X, Zhang X, Leathers R, Makino A, Huang C, Parsa P, Marcias J, Yuan JX, Jamieson SW, Thistlethwaite PA. Notch3 signaling promotes the development of pulmonary arterial hypertension. Nat Med. 15(11):1289-1297. 2009. PMCID: PMC2780347.
  46. Xu M, Remillard CV, Sachs BD, Makino A, Platoshyn O, Yao W, Dillmann WH, Akassoglou K, Yuan JX. p75 Neurotrophin receptor regulates agonist-induced pulmonary vasoconstriction. Am J Physiol Heart Circ Physiol. 295:H1529-H1538. 2008. PMCID: PMC2593496.
  47. Xu M, Platoshyn O, Makino A, Dillmann WH, Akassoglou K, Remillard CV, Yuan JX. Characterization of agonist-induced vasoconstriction in mouse pulmonary artery. Am J Physiol Heart Circ Physiol. 294:H220-H228. 2008. PMID: 17982012.

Thyroid Disease

  1. Suarez J, Wang H, Scott BT, Ling H, Makino A, Swanson E, Brown JH, Suarez JA, Feinstein S, Diaz-Juarez J, Dillmann WH. In vivo selective expression of thyroid hormone receptor α1 in endothelial cells attenuates myocardial injury in experimental myocardial infarction in mice. Am J Physiol Regul Integr Comp Physiol. 2014 Aug 1;307(3):R340-6. doi: 10.1152/ajpregu.00449.2013. Epub 2014 May 21. PMID: 24848360; PMCID: PMC4121632.
  2. Makino A, Wang H, Scott BT, Yuan JX, Dillmann WH. Thyroid hormone receptor-α and vascular function. Am J Physiol Cell Physiol. 2012 May 1;302(9):C1346-52. doi: 10.1152/ajpcell.00292.2011. Epub 2012 Feb 8. PMID: 22322976; PMCID: PMC3361953.
  3. Makino A, Suarez J, Wang H, Belke DD, Scott BT, Dillmann WH. Thyroid hormone receptor-beta is associated with coronary angiogenesis during pathological cardiac hypertrophy. Endocrinology. 2009 Apr;150(4):2008-15. doi: 10.1210/en.2008-0634. Epub 2008 Dec 12. PMID: 19074585; PMCID: PMC2659277.

Renal Flow Regulation in Hypertension

  1. Makino A, Skelton MM, Zou AP, Cowley AW Jr. Increased renal medullary H2O2 leads to hypertension. Hypertension. 2003 Jul;42(1):25-30. doi: 10.1161/01.HYP.0000074903.96928.91. Epub 2003 Jun 2. PMID: 12782642.
  2. Makino A, Skelton MM, Zou AP, Roman RJ, Cowley AW Jr. Increased renal medullary oxidative stress produces hypertension. Hypertension. 2002 Feb;39(2 Pt 2):667-72. doi: 10.1161/hy0202.103469. PMID: 11882628.

Kawasaki Disease

  1. Gu W, Mirsaidi-Madjdabadi S, Ramirez F Jr, Simonson TS, Makino A. Transcriptome meta-analysis of Kawasaki disease in humans and mice. Front Pediatr. 2024 Sep 16;12:1423958. doi: 10.3389/fped.2024.1423958. PMID: 39350793; PMCID: PMC11440715.

Cellular Response to Mechanical Stimulation

  1. Shentu TP, Singh DK, Oh MJ, Sun S, Sadaat L, Makino A, Mazzone T, Subbaiah PV, Cho M, Levitan I. The role of oxysterols in control of endothelial stiffness. J Lipid Res. 2012 Jul;53(7):1348-58. doi: 10.1194/jlr.M027102. Epub 2012 Apr 11. PMID: 22496390; PMCID: PMC3371246.
  2. Song S, Yamamura A, Yamamura H, Ayon RJ, Smith KA, Tang H, Makino A, Yuan JX. Flow shear stress enhances intracellular Ca2+ signaling in pulmonary artery smooth muscle cells from patients with pulmonary arterial hypertension. Am J Physiol Cell Physiol. 2014 Aug 15;307(4):C373-83. doi: 10.1152/ajpcell.00115.2014. Epub 2014 Jun 11. PMID: 24920677; PMCID: PMC4137136.
  3. Makino A, Prossnitz ER, Bünemann M, Wang JM, Yao W, Schmid-Schönbein GW. G protein-coupled receptors serve as mechanosensors for fluid shear stress in neutrophils. Am J Physiol Cell Physiol. 2006 Jun;290(6):C1633-9. doi: 10.1152/ajpcell.00576.2005. Epub 2006 Jan 25. PMID: 16436471.
  4. Makino A, Glogauer M, Bokoch GM, Chien S, Schmid-Schönbein GW. Control of neutrophil pseudopods by fluid shear: role of Rho family GTPases. Am J Physiol Cell Physiol. 2005 Apr;288(4):C863-71. doi: 10.1152/ajpcell.00358.2004. Epub 2004 Nov 23. PMID: 15561759.

Review Articles

  1. Cowley AW Jr, Roman RJ, Mattson DL, Franchini KG, O’Connor PM, Makino A, Taylor NE, Evans LC, Mori T, Dickhout JG, Jin C, Miyata N, Nakanishi K, Szentiványi M Jr, Park F, Skelton MM, Kurth T, Shimada S. Renal Medulla in Hypertension. Hypertension. 2024 Dec;81(12):2383-2394. doi: 10.1161/HYPERTENSIONAHA.124.21711. Epub 2024 Sep 30. PMID: 39344517; PMCID: PMC11578791.
  2. Balistrieri A, Makino A, Yuan JX. Pathophysiology and pathogenic mechanisms of pulmonary hypertension: role of membrane receptors, ion channels, and Ca2+ signaling. Physiol Rev. 2023 Jul 1;103(3):1827-1897. doi: 10.1152/physrev.00030.2021. Epub 2022 Nov 24. PMID: 36422993; PMCID: PMC10110735.
  3. Cabrera JTO, Makino A. Efferocytosis of vascular cells in cardiovascular disease. Pharmacol Ther. 2022 Jan;229:107919. doi: 10.1016/j.pharmthera.2021.107919. Epub 2021 Jun 23. PMID: 34171333; PMCID: PMC8695637.
  4. Babicheva A, Makino A, Yuan JX. mTOR Signaling in Pulmonary Vascular Disease: Pathogenic Role and Therapeutic Target. Int J Mol Sci. 2021 Feb 21;22(4):2144. doi: 10.3390/ijms22042144. PMID: 33670032; PMCID: PMC7926633.
  5. Willson C, Watanabe M, Tsuji-Hosokawa A, Makino A. Pulmonary vascular dysfunction in metabolic syndrome. J Physiol. 2019 Feb;597(4):1121-1141. doi: 10.1113/JP275856. Epub 2018 Sep 12. PMID: 30125956; PMCID: PMC6375868.
  6. Truong, A.H., Murugesan, S., Youssef, K.D., Makino, A. (2016). Mitochondrial Ion Channels in Metabolic Disease. In: Levitan, PhD, I., Dopico, MD, PhD, A. (eds) Vascular Ion Channels in Physiology and Disease. Springer, Cham. https://doi.org/10.1007/978-3-319-29635-7_18
  7. Smith KA, Ayon RJ, Tang H, Makino A, Yuan JX. Calcium-Sensing Receptor Regulates Cytosolic [Ca 2+ ] and Plays a Major Role in the Development of Pulmonary Hypertension. Front Physiol. 2016 Nov 4;7:517. doi: 10.3389/fphys.2016.00517. PMID: 27867361; PMCID: PMC5095111.
  8. Pangare M, Makino A. Mitochondrial function in vascular endothelial cell in diabetes. J Smooth Muscle Res. 2012;48(1):1-26. doi: 10.1540/jsmr.48.1. PMID: 22504486; PMCID: PMC3655204.
  9. Song MY, Makino A, Yuan JX. Role of reactive oxygen species and redox in regulating the function of transient receptor potential channels. Antioxid Redox Signal. 2011 Sep 15;15(6):1549-65. doi: 10.1089/ars.2010.3648. Epub 2011 Apr 11. PMID: 21126186; PMCID: PMC3151422.
  10. Makino A, Firth AL, Yuan JX. Endothelial and smooth muscle cell ion channels in pulmonary vasoconstriction and vascular remodeling. Compr Physiol. 2011 Jul;1(3):1555-602. doi: 10.1002/cphy.c100023. PMID: 23733654; PMCID: PMC5524522.
  11. Hainsworth Y. Shin, Xiaoyan Zhang, Ayako Makino, Geert W. Schmid-Schönbein. The mechanobiological evidence for the control of neutrophil activity by fluid shear stress. Book: Mechanobiology Handbook. Chapter 8, 139-175, 2011.
  12. Remillard, C.V., Makino, A., Yuan, J.XJ. (2011). Isolation and Culture of Pulmonary Vascular Smooth Muscle and Endothelial Cells. In: Yuan, JJ., Garcia, J., West, J., Hales, C., Rich, S., Archer, S. (eds) Textbook of Pulmonary Vascular Disease. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-87429-6_33
  13. Makino A, Shin HY, Komai Y, Fukuda S, Coughlin M, Sugihara-Seki M, Schmid-Schönbein GW. Mechanotransduction in leukocyte activation: a review. Biorheology. 2007;44(4):221-49. PMID: 18094448.

Methodological Articles

  1. Luo S, Truong AH, Makino A. Isolation of Mouse Coronary Endothelial Cells. J Vis Exp. 2016 Jul 3;(113):53985. doi: 10.3791/53985. PMID: 27404385; PMCID: PMC4993319.